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KMID : 0381120220440070813
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Genes and Genomics
2022 Volume.44 No. 7 p.813 ~ p.822
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Comprehensive analysis of TCR repertoire of COVID-19 patients in different infected stage
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Wang Guangyu
Wang Yongsi
Jiang Shaofeng
Fan Wentao
Mo Chune
Gong Weiwei
Chen Hui
He Dan
Huang Jinqing
Ou Minglin
Hou Xianliang
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Abstract
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Background: The current pandemic of coronavirus disease 2019 (COVID-19), transmitted person-to-person by the severe acute respiratory syndrome of coronavirus 2 (SARS-CoV-2), poses a threat to global public health.
Objective: In this study, we performed the comprehensive analysis of the T cell receptor (TCR) repertoire may contribute to a more in-depth understanding of the pathogenesis of COVID-19.
Methods: A comprehensive immunological analysis was performed to explore the features of the TCR repertoire and identified TCR sequences correlated with SARS-CoV-2 viral antigens.
Results: we analyzed the COVID-19 patients’ TCR repertoires in peripheral blood mononuclear cells (PBMC) which obtained before (baseline), during (acute), and after rehabilitation (convalescent) by ImmunoSEQ-technology, and found that repertoire features of TCRβ-chain (TCRβ) complementary-determining region 3 (CDR3) in COVID-19 patients were remarkable difference, including decreased TCR diversity, abnormal CDR3 length, difference of TRBV/J gene usage and higher TCR sequence overlap. Besides, we identified some COVID-19 disease-associated TCRβ clones, and the abundance of them changed with the progression of the disease. Importantly, these disease-associated TCRβ clones could be used to distinguish COVID-19 patients from healthy controls with high accuracy.
Conclusions: We provide a clear understanding of the TCR repertoire of COVID-19 patients, which lays the foundation for better diagnosis and treatment of COVID-19 patients.
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KEYWORD
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Coronavirus disease 2019, SARS-CoV-2, T cell receptor
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